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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB8 Recombinant antibody (86241-1-RR)

    WB analysis using 86241-1-RR (same clone as 86241-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86241-1-RR (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86241-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMB8 Recombinant antibody (86241-1-RR)

    IF Staining of HeLa using 86241-1-RR (same clone as 86241-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB8 antibody (86241-1-RR, Clone: 250865C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86241-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86241-1-RR

    Affinity and Kinetic Characterization of 86241-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86241-1-RR against Human PSMB8 were performed. The affinity constant is below 1 pM.

PSMB8 Recombinant monoclonal antibody, PBS Only

PSMB8 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86241-1-PBS
Clone No.250865C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:3.4.25.1, LMP7, Proteasome subunit beta type 8, Proteasome subunit beta type-8, Proteasome subunit beta-5i

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86241-1-PBS targets PSMB8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6660

Product name: Recombinant human PSMB8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC001114

Sequence: MLIGTPTPRDTTPSSWLTSSLLVEAAPLDDTTLPTPVSSGCPGLEPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Calculated molecular weight 30 kDa
Observed molecular weight 23 kDa, 30 kDa
GenBank accession numberBC001114
Gene Symbol PSMB8
Gene ID (NCBI) 5696
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP28062
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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