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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB8 Recombinant antibody (86241-1-RR)

    WB analysis using 86241-1-RR (same clone as 86241-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86241-1-RR (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86241-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMB8 Recombinant antibody (86241-1-RR)

    IF Staining of HeLa using 86241-1-RR (same clone as 86241-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB8 antibody (86241-1-RR, Clone: 250865C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86241-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86241-1-RR

    Affinity and Kinetic Characterization of 86241-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86241-1-RR against Human PSMB8 were performed. The affinity constant is below 1 pM.

PSMB8 Recombinant monoclonal antibody, PBS Only

PSMB8 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86241-1-PBS
Clone No.250865C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:3.4.25.1, LMP7, Proteasome subunit beta type 8, Proteasome subunit beta type-8, Proteasome subunit beta-5i

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86241-1-PBS targets PSMB8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6660

Product name: Recombinant human PSMB8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC001114

Sequence: MLIGTPTPRDTTPSSWLTSSLLVEAAPLDDTTLPTPVSSGCPGLEPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Calculated molecular weight 30 kDa
Observed molecular weight 23 kDa, 30 kDa
GenBank accession numberBC001114
Gene Symbol PSMB8
Gene ID (NCBI) 5696
RRIDAB_3744743
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP28062
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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