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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMB7 Monoclonal antibody

PSMB7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D7B8

Cat no : 68641-1-Ig

Synonyms

Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7, Z

Validation Data Gallery

Applications:
  • WB analysis using 68641-1-Ig

    WB analysis using 68641-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68641-1-Ig (PSMB7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, LNCaP cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PSMB7 Ready-To-Use IHC Kit. PSMB7 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68641-1-Ig targets PSMB7 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMB7 fusion protein Ag6544 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 7
Calculated molecular weight29.9 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC000509
Gene symbol PSMB7
Gene ID (NCBI) 5695
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)

Protocols

Product Specific Protocols
WB protocol for PSMB7 antibody 68641-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols