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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB7 Polyclonal antibody (30283-1-AP)

    WB analysis using 30283-1-AP (same clone as 30283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PSMB7 Polyclonal antibody (30283-1-AP)

    WB analysis using 30283-1-AP (same clone as 30283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB7 Polyclonal antibody (30283-1-AP)

    IHC staining of human colon cancer using 30283-1-AP (same clone as 30283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB7 Polyclonal antibody (30283-1-AP)

    IHC staining of human colon cancer using 30283-1-AP (same clone as 30283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30283-1-PBS targets PSMB7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMB7 fusion protein Ag32201 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 7
Calculated molecular weight29.9 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC000509
Gene Symbol PSMB7
Gene ID (NCBI) 5695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99436
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)