Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, A549 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
14727-1-AP targets PSMB7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6486 Product name: Recombinant human PSMB7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-277 aa of BC000509 Sequence: MAAVSVYAPPVGGFSFDNCRRNAVLEADFAKRGYKLPKARKTGTTIAGVVYKDGIVLGADTRATEGMVVADKNCSKIHFISPNIYCCGAGTAADTDMTTQLISSNLELHSLSTGRLPRVVTANRMLKQMLFRYQGYIGAALVLGGVDVTGPHLYSIYPHGSTDKLPYVTMGSGSLAAMAVFEDKFRPDMEEEEAKNLVSEAIAAGIFNDLGSGSNIDLCVISKNKLDFLRPYTVPNKKGTRLGRYRCEKGTTAVLTEKITPLEIEVLEETVQTMDTS 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) subunit, beta type, 7 |
| Calculated molecular weight | 29.9 kDa |
| Observed molecular weight | 25-27 kDa |
| GenBank accession number | BC000509 |
| Gene Symbol | PSMB7 |
| Gene ID (NCBI) | 5695 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99436 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSMB7 antibody 14727-1-AP | Download protocol |
| WB protocol for PSMB7 antibody 14727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction. | ||
Int J Mol Sci Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats. | ||
Biochem Pharmacol Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP) |