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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB3 Polyclonal antibody (15983-1-AP)

    WB analysis using 15983-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMB3 Polyclonal antibody (15983-1-AP)

    WB analysis using 15983-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMB3 Polyclonal antibody (15983-1-AP)

    WB analysis using 15983-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PSMB3 Polyclonal antibody (15983-1-AP)

    IHC staining of human liver using 15983-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15983-1-AP (PSMB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using PSMB3 Polyclonal antibody (15983-1-AP)

    IHC staining of human liver using 15983-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15983-1-AP (PSMB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PSMB3 Polyclonal antibody (15983-1-AP)

    IF Staining of Jurkat using 15983-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMB3 antibody (15983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

PSMB3 Polyclonal antibody

PSMB3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15983-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Proteasome subunit beta type-3, Proteasome component C10-II, HC10 II

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HEK-293T cells, mouse testis tissue, rat testis tissue, HeLa cells, MCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15983-1-AP targets PSMB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8784

Product name: Recombinant human PSMB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-205 aa of BC013008

Sequence: MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRFGPYYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCAEQMYGMCESLWEPNMDPDHLFETISQAMLNAVDRDAVSGMGVIVHIIEKDKITTRTLKARMD

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 3
Calculated molecular weight 205 aa, 23 kDa
Observed molecular weight 23-27 kDa
GenBank accession numberBC013008
Gene Symbol PSMB3
Gene ID (NCBI) 5691
RRIDAB_2172027
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49720
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PSMB3 antibody 15983-1-APDownload protocol
IHC protocol for PSMB3 antibody 15983-1-APDownload protocol
WB protocol for PSMB3 antibody 15983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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