Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
Product Information
15154-1-AP targets PSMB2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7198 Product name: Recombinant human PSMB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC000268 Sequence: MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQGS 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) subunit, beta type, 2 |
| Calculated molecular weight | 23 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC000268 |
| Gene Symbol | PSMB2 |
| Gene ID (NCBI) | 5690 |
| RRID | AB_2300322 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49721 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMB2, Proteasome subunit beta type-2, is also known as 20S proteasome subunit beta-4(PMID: 7918633). The proteasome is responsible for degradation of short lived and misfolded cytosolic and nuclear proteins in the cell and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMB2 is up-regulated in ovarian cancer cell lines. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD (vitamin A depletion) brain regions (PMID: 21190828). Knockdown of PSMB2 suppresses hepatocellular carcinoma and gastric cancer cell proliferation and invasion (PMID: 29780166, PMID: 36110152).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSMB2 antibody 15154-1-AP | Download protocol |
| WB protocol for PSMB2 antibody 15154-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Med Suppressing proteasome activity enhances sensitivity to actinomycin D in diffuse anaplastic Wilms tumor | ||
Phytomedicine PTEN status determines therapeutic vulnerability to celastrol in cholangiocarcinoma | ||
Oncogene NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.
| ||
Cell Mol Neurobiol Inhibition of the Proteasome Regulator PA28 Aggravates Oxidized Protein Overload in the Diabetic Rat Brain | ||
PLoS Biol Pharmacological induction of autophagy reduces inflammation in macrophages by degrading immunoproteasome subunits | ||
Mol Cancer Res Proteasome subunits differentially control Myeloma cell viability and proteasome inhibitor sensitivity. |