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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis using 66403-1-Ig (same clone as 66403-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of Raji using 66403-1-Ig (same clone as 66403-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat thymus tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of rat thymus using 66403-1-Ig (same clone as 66403-1-PBS)

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of Ramos using 66403-1-Ig (same clone as 66403-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of U-937 using 66403-1-Ig (same clone as 66403-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of human spleen using 66403-1-Ig (same clone as 66403-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig thymus tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of pig thymus using 66403-1-Ig (same clone as 66403-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    IHC staining of mouse kidney using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PSMB10 Monoclonal antibody (66403-1-Ig)

    IF Staining of Jurkat using 66403-1-Ig (same clone as 66403-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMB10 antibody (66403-1-Ig, Clone: 1G8C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

PSMB10 Monoclonal antibody, PBS Only

PSMB10 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66403-1-PBS
Clone No.1G8C1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Multicatalytic endopeptidase complex subunit MECl-1, MECL1, Macropain subunit MECl-1, LMP10, EC:3.4.25.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66403-1-PBS targets PSMB10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8835

Product name: Recombinant human PSMB10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-273 aa of BC017198

Sequence: MLKPALEPRGGFSFENCQRNASLERVLPGLKVPHARKTGTTIAGLVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 10
Calculated molecular weight 273 aa, 29 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC017198
Gene Symbol PSMB10
Gene ID (NCBI) 5699
RRIDAB_2881777
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP40306
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SMB10(Proteasome subunit beta type-10) is also named as LMP10, MECL1.It belongs to the peptidase T1B family and is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.