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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA7 Monoclonal antibody (67817-1-Ig)

    WB analysis using 67817-1-Ig (same clone as 67817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67817-1-Ig (PSMA7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PSMA7 Monoclonal antibody (67817-1-Ig)

    WB analysis of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

    WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected MCF-7 cells. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PSMA7 Monoclonal antibody (67817-1-Ig)

    WB analysis of HeLa using 67817-1-Ig (same clone as 67817-1-PBS)

    WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected HeLa cells. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PSMA7 Monoclonal antibody (67817-1-Ig)

    IHC staining of human cervical cancer using 67817-1-Ig (same clone as 67817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PSMA7 Monoclonal antibody (67817-1-Ig)

    IHC staining of human cervical cancer using 67817-1-Ig (same clone as 67817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PSMA7 Monoclonal antibody (67817-1-Ig)

    IF Staining of Caco-2 using 67817-1-Ig (same clone as 67817-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PSMA7 Monoclonal antibody (67817-1-Ig)

    IF Staining of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PSMA7 Monoclonal antibody (67817-1-Ig)

    FC experiment of HeLa using 67817-1-Ig (same clone as 67817-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug PSMA7 Monoclonal antibody (67817-1-Ig, Clone:1F2C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PSMA7 Monoclonal antibody, PBS Only

PSMA7 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67817-1-PBS
Clone No.1F2C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

C6, HSPC, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, RC6 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67817-1-PBS targets PSMA7 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7821

Product name: Recombinant human PSMA7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-248 aa of BC004427

Sequence: MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 7
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC004427
Gene Symbol PSMA7
Gene ID (NCBI) 5688
RRIDAB_2918580
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO14818
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).

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