Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, mouse liver tissue, mouse thymus tissue |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
31384-1-AP targets PSMA5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSMA5 fusion protein Ag34928 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) subunit, alpha type, 5 |
| Calculated molecular weight | 241 aa, 26 kDa |
| Observed molecular weight | 26-30 kDa |
| GenBank accession number | BC103751 |
| Gene Symbol | PSMA5 |
| Gene ID (NCBI) | 5686 |
| RRID | AB_3669959 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P28066 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMA5 antibody 31384-1-AP | Download protocol |
| IF protocol for PSMA5 antibody 31384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|