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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA5 Polyclonal antibody (30580-1-AP)

    WB analysis using 30580-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30580-1-AP (PSMA5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PSMA5 Polyclonal antibody (30580-1-AP)

    IHC staining of mouse testis using 30580-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30580-1-AP (PSMA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA5 Polyclonal antibody (30580-1-AP)

    IF Staining of HeLa using 30580-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (30580-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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PSMA5 Polyclonal antibody

PSMA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30580-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ZETA, PSC5, Proteasome subunit alpha type-5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HCT 116 cells, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30580-1-AP targets PSMA5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMA5 fusion protein Ag30703 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 5
Calculated molecular weight 26 kDa
Observed molecular weight28 kDa
GenBank accession numberNM_002790
Gene Symbol PSMA5
Gene ID (NCBI) 5686
RRIDAB_3086368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

Protocols

Product Specific Protocols
WB protocol for PSMA5 antibody 30580-1-APDownload protocol
IHC protocol for PSMA5 antibody 30580-1-APDownload protocol
IF protocol for PSMA5 antibody 30580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols