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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using PSMA3 Polyclonal antibody (11887-1-AP)

    WB analysis of A549 using 11887-1-AP (same clone as 11887-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human lung tissue using PSMA3 Polyclonal antibody (11887-1-AP)

    WB analysis of human lung using 11887-1-AP (same clone as 11887-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using PSMA3 Polyclonal antibody (11887-1-AP)

    WB analysis of PC-12 using 11887-1-AP (same clone as 11887-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PSMA3 Polyclonal antibody (11887-1-AP)

    WB analysis using 11887-1-AP (same clone as 11887-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PSMA3 Polyclonal antibody (11887-1-AP)

    IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PSMA3 Polyclonal antibody (11887-1-AP)

    IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMA3 Polyclonal antibody (11887-1-AP)

    IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMA3 Polyclonal antibody (11887-1-AP)

    IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PSMA3 Polyclonal antibody (11887-1-AP)

    IHC staining of mouse brain using 11887-1-AP (same clone as 11887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA3 Polyclonal antibody (11887-1-AP)

    IF Staining of HeLa using 11887-1-AP (same clone as 11887-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA3 antibody (11887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

PSMA3 Polyclonal antibody, PBS Only

PSMA3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11887-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PSC8, Proteasome subunit alpha type-3, HC8

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11887-1-PBS targets PSMA3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2495

Product name: Recombinant human PSMA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-255 aa of BC029402

Sequence: MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADMAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDESDDDNM

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 3
Calculated molecular weight 255 aa, 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC029402
Gene Symbol PSMA3
Gene ID (NCBI) 5684
RRIDAB_2171420
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP25788
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

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