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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA1 Monoclonal antibody (67910-1-Ig)

    WB analysis using 67910-1-Ig (same clone as 67910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using PSMA1 Monoclonal antibody (67910-1-Ig)

    WB analysis of RAW 264.7 using 67910-1-Ig (same clone as 67910-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using PSMA1 Monoclonal antibody (67910-1-Ig)

    WB analysis of 4T1 using 67910-1-Ig (same clone as 67910-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMA1 Monoclonal antibody (67910-1-Ig)

    IHC staining of human colon cancer using 67910-1-Ig (same clone as 67910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67910-1-Ig (PSMA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMA1 Monoclonal antibody (67910-1-Ig)

    IHC staining of human colon cancer using 67910-1-Ig (same clone as 67910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67910-1-Ig (PSMA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

PSMA1 Monoclonal antibody, PBS Only

PSMA1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67910-1-PBS
Clone No.1D1D5

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

30 kDa prosomal protein, HC2, Macropain subunit C2, NU, PROS 30, PROS30, Proteasome component C2, Proteasome nu chain, PSC2, PSMA1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67910-1-PBS targets PSMA1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30527

Product name: Recombinant human PSMA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-263 aa of BC015105

Sequence: MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPAQPADEPAEKADEPMEH

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 1
Calculated molecular weight 30 kDa
Observed molecular weight 29-33 kDa
GenBank accession numberBC015105
Gene Symbol PSMA1
Gene ID (NCBI) 5682
RRIDAB_2918665
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP25786
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PSMA1(Proteasome subunit alpha type-1) is also named as HC2, NU, PROS30, PSC2 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It is induced in breast cancer tissue and up-regulated in liver tumor tissues(PMID:17127214;16317774;17004105).