• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSKH2 Polyclonal antibody

PSKH2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18813-1-AP

Synonyms

protein serine kinase H2, PSK H2, PSKH2

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 18813-1-AP

    WB analysis of HepG2 using 18813-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 18813-1-AP

    WB analysis of human heart using 18813-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 18813-1-AP

    WB analysis of human brain using 18813-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 18813-1-AP

    WB analysis of human testis using 18813-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 18813-1-AP

    IP experiment of HepG2 using 18813-1-AP

    IP result of anti-PSKH2 (IP:18813-1-AP, 4ug; Detection:18813-1-AP 1:500) with HepG2 cells lysate 3400ug.

  • IHC staining of human kidney using 18813-1-AP

    IHC staining of human kidney using 18813-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 18813-1-AP

    IHC staining of human kidney using 18813-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 18813-1-AP

    IF Staining of HepG2 using 18813-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18813-1-AP (PSKH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, human heart tissue, human testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18813-1-AP targets PSKH2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein serine kinase H2
Calculated molecular weight 43 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC126182
Gene symbol PSKH2
Gene ID (NCBI) 85481
RRIDAB_2171244
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSKH2 belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. PSKH2 catalytic the reaction: ATP + a protein = ADP + a phosphoprotein.

Protocols

Product Specific Protocols
WB protocol for PSKH2 antibody 18813-1-APDownload protocol
IHC protocol for PSKH2 antibody 18813-1-APDownload protocol
IF protocol for PSKH2 antibody 18813-1-APDownload protocol
IP protocol for PSKH2 antibody 18813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols