Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87878-3-RR targets PSG8 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg8783 Product name: Recombinant human PSG8 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 35-426 aa of NM_182707.3 Sequence: QVTIEAQPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQIRDLYHYITSYVVDGQIIIYGPAYSGRETIYSNASLLIQNVTQEDAGSYTLHIIMGGDENRGVTGHFTFTLYLETPKPSISSSKLNPREAMEAVSLTCDPETPDASYLWWMNGQSLPMSHRLQLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPFTLNLLPKLPKPYITINNLKPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDQYGGIRSYPVTLNVLYGPDLPRIYPSFTYYRSGEVLYLSCSADSNPPAQYSWTINGKFQLSGQKLFIPQITTKHSGLYACSVRNSATGKESSKSMTVKVSGKRIPVSLAIGI 相同性解析による交差性が予測される生物種 |
| Full Name | pregnancy specific beta-1-glycoprotein 8 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 45-75 kDa |
| GenBank accession number | NM_182707.3 |
| Gene Symbol | PSG8 |
| Gene ID (NCBI) | 440533 |
| RRID | AB_3745803 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UQ74-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pregnancy-specific β1-glycoproteins (PSGs) are predominant proteins synthesized during gestation in humans, primates and rodents (PMID: 5001318; 11133662). In humans, PSGs are mainly secreted by syncytiotrophoblasts, while in rodents they derive from trophoblast giant cells and spongiotrophoblasts (PMID: 9290143). Moreover, PSGs can serve as indicators for blastocyst quality and embryonic viability (PMID: 8671387; 2409861).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSG8 antibody 87878-3-RR | Download protocol |
| WB protocol for PSG8 antibody 87878-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |