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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSG3 Polyclonal antibody (15375-1-AP)

    WB analysis using 15375-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15375-1-AP (PSG3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG3 Polyclonal antibody (15375-1-AP)

    IHC staining of human placenta using 15375-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15375-1-AP (PSG3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG3 Polyclonal antibody (15375-1-AP)

    IHC staining of human placenta using 15375-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15375-1-AP (PSG3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSG3 Polyclonal antibody

PSG3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15375-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Carcinoembryonic antigen SG5, Pregnancy-specific beta-1-glycoprotein 3, Pregnancy-specific glycoprotein 3, PS beta G 3, PS-beta-G-3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15375-1-AP targets PSG3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1148

Product name: Recombinant human PSG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 129-428 aa of BC005924

Sequence: DGTRGETGHFTFTLYLETPKPSISSSNLYPREDMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLQLSKNKRTLFLFGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLAFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIQDRYGGIRSYPVTLNVLYGPDLPRIYPSFTYYHSGENLYLSCFADSNPPAEYSWTINGKFQLSGQKLFIPQITTKHSGLYACSVRNSATGMESSKSMTVEVSAPSGTGHLPGLNPL

相同性解析による交差性が予測される生物種
Full Name pregnancy specific beta-1-glycoprotein 3
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa, 64 kDa
GenBank accession numberBC005924
Gene Symbol PSG3
Gene ID (NCBI) 5671
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16557
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human pregnancy-specific glycoproteins (PSGs) are a group of molecules that are mainly produced by the placental syncytiotrophoblasts during pregnancy (PMID: 1644821). The calculated molecular weight of PSG3 is 48 kDa. The 64 kDa band is its glycosylated protein form (PMID: 7851896).

Protocols

Product Specific Protocols
WB protocol for PSG3 antibody 15375-1-APDownload protocol
IHC protocol for PSG3 antibody 15375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols