Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of mouse uterus using 16352-1-AP (same clone as 16352-1-PBS)
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 16352-1-AP (PSG11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16352-1-AP (same clone as 16352-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16352-1-AP (PSG11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16352-1-AP (same clone as 16352-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 16352-1-AP (same clone as 16352-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSG11 antibody (16352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16352-1-PBS in a different storage buffer formulation.

PSG11 Polyclonal antibody, PBS Only

PSG11 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16352-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Pregnancy-specific beta-1-glycoprotein 11, Pregnancy-specific beta-1-glycoprotein 13, Pregnancy-specific glycoprotein 11, Pregnancy-specific glycoprotein 13, PS beta G 11

Formulation: PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16352-1-PBS targets PSG11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9562 Product name: Recombinant human PSG11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC020711 Sequence: MHAAEIMGPLSAPPCTEHIKWKGLLLTVETPKPSISSSNLNPREAMETVILTCNPETPDASYLWWMNGQSLPMTHRMQLSETNRTLFLFGVTKYTAGPYECEIWNSGSASRSDPVTLNLLHGPDLPRIFPSVTSYYSGENLDLSCFADSNPPAQYSWTINGKFQLSGQKLFIPQITPKHNGLYACSARNSATGEESSTSLTIRVIAPPGLGTFAFNNPT 相同性解析による交差性が予測される生物種
Full Name	pregnancy specific beta-1-glycoprotein 11
Calculated molecular weight	219 aa, 24 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC020711
Gene Symbol	PSG11
Gene ID (NCBI)	5680
RRID	AB_2171065
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UQ72
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

PSG11, also named as PSG13, PSG14, PSBG-11 and PSBG-13, belongs to the immunoglobulin superfamily and CEA family. PSBG are produced in high quantity during pregnancy. PSG11 and other PSGs induce dose-dependent secretion of anti-inflammatory cytokines by human monocytes. (PMID:11327547)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse uterus using 16352-1-AP (same clone as 16352-1-PBS)

WB analysis of HeLa using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human brain using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human brain using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human liver using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human liver using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human lung using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human lung using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human kidney using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human kidney using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human placenta using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human placenta using 16352-1-AP (same clone as 16352-1-PBS)

IHC staining of human testis using 16352-1-AP (same clone as 16352-1-PBS)