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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using PSG1 Polyclonal antibody (11809-1-AP)

    WB analysis of human kidney using 11809-1-AP (same clone as 11809-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11809-1-AP (PSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11809-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PSG1 Polyclonal antibody (11809-1-AP)

    WB analysis of MCF-7 using 11809-1-AP (same clone as 11809-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11809-1-AP (PSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG1 Polyclonal antibody (11809-1-AP)

    IHC staining of human placenta using 11809-1-AP (same clone as 11809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG1 Polyclonal antibody (11809-1-AP)

    IHC staining of human placenta using 11809-1-AP (same clone as 11809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11809-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PSG1 Polyclonal antibody (11809-1-AP)

    IF Staining of MCF-7 using 11809-1-AP (same clone as 11809-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSG1 antibody (11809-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11809-1-PBS in a different storage buffer formulation.

PSG1 Polyclonal antibody, PBS Only

PSG1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11809-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FL NCA 1/2, Fetal liver non-specific cross-reactive antigen 1/2, DHFRP2, CD66f, CD66 antigen-like family member F

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11809-1-PBS targets PSG1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2468

Product name: Recombinant human PSG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 35-349 aa of BC022338

Sequence: QVTIEAEPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQMRDLYHYITSYVVDGEIIIYGPAYSGRETAYSNASLLIQNVTREDAGSYTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYRSGEV

相同性解析による交差性が予測される生物種
Full Name pregnancy specific beta-1-glycoprotein 1
Calculated molecular weight 426 aa, 48 kDa
Observed molecular weight 72 kDa, 36 kDa
GenBank accession numberBC022338
Gene Symbol PSG1
Gene ID (NCBI) 5669
RRIDAB_2171045
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11464
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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