Validation Data Gallery
Tested Applications
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | mouse brain tissue, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
20434-1-AP targets PSEN1 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14135 Product name: Recombinant human PSEN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 254-377 aa of BC011729 Sequence: LVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAAVQELSSSILAGEDPEERGVKL 相同性解析による交差性が予測される生物種 |
| Full Name | presenilin 1 |
| Calculated molecular weight | 467 aa, 53 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC011729 |
| Gene Symbol | PSEN1 |
| Gene ID (NCBI) | 5663 |
| RRID | AB_2878685 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49768 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a ~30-kDa N-terminal fragment (NTF) and a ~20-kDa C-terminal fragment (CTF) by the 26S proteasome(PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody can recognize all the isoforms except isoform 4 (21 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSEN1 antibody 20434-1-AP | Download protocol |
| IHC protocol for PSEN1 antibody 20434-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
