Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10501-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10501-1-AP (PSAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10501-1-AP
WB result of PSAT1 antibody (10501-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAT1 transfected HEK-293 cells.
WB analysis of Jurkat using 10501-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10501-1-AP (PSAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10501-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10501-1-AP (PSAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10501-1-AP
IP result of anti-PSAT1 (IP:10501-1-AP, 3ug; Detection:10501-1-AP 1:300) with HeLa cells lysate 2480ug.
IHC staining of human pancreas cancer using 10501-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10501-1-AP (PSAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10501-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10501-1-AP (PSAT1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 10501-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10501-1-AP (PSAT1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 10501-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAT1 antibody (10501-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of HepG2 using 10501-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAT1 antibody (10501-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 10501-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSAT1 Polyclonal antibody (10501-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

PSAT1 Polyclonal antibody

PSAT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10501-1-AP

Publications(85)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:2.6.1.52, EPIP, Phosphoserine aminotransferase, PSA, PSAT

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (85)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, HEK-293 cells, HepG2 cells, K-562 cells, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 10 publications below
WB	See 80 publications below
IHC	See 15 publications below
IF	See 4 publications below
CoIP	See 2 publications below

Product Information

10501-1-AP targets PSAT1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0776 Product name: Recombinant human PSAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-370 aa of BC004863 Sequence: MDAPRQVVNFGPGPAKLPHSVLLEIQKELLDYKGVGISVLEMSHRSSDFAKIINNTENLVRELLAVPDNYKVIFLQGGGCGQFSAVPLNLIGLKAGRCADYVVTGAWSAKAAEEAKKFGTINIVHPKLGSYTKIPDPSTWNLNPDASYVYYCANETVHGVEFDFIPDVKGAVLVCDMSSNFLSKPVDVSKFGVIFAGAQKNVGSAGVTVVIVRDDLLGFALRECPSVLEYKVQAGNSSLYNTPPCFSIYVMGLVLEWIKNNGGAAAMEKLSSIKSQTIYEIIDNSQGFYVCPVEPQNRSKMNIPFRIGNAKGDDALEKRFLDKALELNMLSLKGHRSVGGIRASLYNAVTIEDVQKLAAFMKKFLEMHQL 相同性解析による交差性が予測される生物種
Full Name	phosphoserine aminotransferase 1
Calculated molecular weight	40 kDa
Observed molecular weight	37-40 kDa
GenBank accession number	BC004863
Gene Symbol	PSAT1
Gene ID (NCBI)	29968
RRID	AB_2172597
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y617
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSAT1, also named PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740)

Protocols

Product Specific Protocols
IF protocol for PSAT1 antibody 10501-1-AP	Download protocol
IHC protocol for PSAT1 antibody 10501-1-AP	Download protocol
IP protocol for PSAT1 antibody 10501-1-AP	Download protocol
WB protocol for PSAT1 antibody 10501-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Stem cell function and stress response are controlled by protein synthesis. Authors - Sandra Blanco View Article
human	WB	Cell Metab Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation. Authors - Long Shen View Article
mouse	WB	Nat Immunol PERK is a critical metabolic hub for immunosuppressive function in macrophages. Authors - Lydia N Raines View Article
mouse	WB	Cell Metab PMID: 28111214 Authors - Eric H Ma View Article
human	WB	Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence Authors - Yinsheng Wu View Article
human	WB	Nat Chem Biol PSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation Authors - Peixiang Zheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10501-1-AP

WB analysis of HEK-293 using 10501-1-AP

WB analysis of Jurkat using 10501-1-AP

WB analysis of HEK-293 using 10501-1-AP

IP experiment of HeLa using 10501-1-AP

IHC staining of human pancreas cancer using 10501-1-AP

IHC staining of human colon cancer using 10501-1-AP

IHC staining of human colon cancer using 10501-1-AP

IF Staining of HepG2 using 10501-1-AP

IF Staining of HepG2 using 10501-1-AP

FC experiment of HepG2 using 10501-1-AP

PSAT1 Polyclonal antibody

PSAT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Stem cell function and stress response are controlled by protein synthesis.

Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation.

PERK is a critical metabolic hub for immunosuppressive function in macrophages.

PMID: 28111214

Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence

PSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation