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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PSAP Polyclonal antibody (18396-1-AP)

    WB analysis of HepG2 using 18396-1-AP

    WB result of PSAP antibody (18396-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

  • Western Blot (WB) analysis of human liver tissue using PSAP Polyclonal antibody (18396-1-AP)

    WB analysis of human liver using 18396-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 18396-1-AP (PSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Polyclonal antibody (18396-1-AP)

    WB analysis of HepG2 using 18396-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18396-1-AP (PSAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18396-1-AP)

    IHC staining of human liver cancer using 18396-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18396-1-AP (PSAP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18396-1-AP)

    IHC staining of human liver cancer using 18396-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18396-1-AP (PSAP antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSAP Polyclonal antibody (18396-1-AP)

    IF Staining of HepG2 using 18396-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18396-1-AP (PSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

PSAP Polyclonal antibody

PSAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18396-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, human liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18396-1-AP targets PSAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13263

Product name: Recombinant human PSAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-143 aa of BC001503

Sequence: LPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQK

相同性解析による交差性が予測される生物種
Full Name prosaposin
Calculated molecular weight 58 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_2172460
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PSAP gene encodes prosaposin, a precursor of fourl small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin A.

Protocols

Product Specific Protocols
IF protocol for PSAP antibody 18396-1-APDownload protocol
IHC protocol for PSAP antibody 18396-1-APDownload protocol
WB protocol for PSAP antibody 18396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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