Validation Data Gallery
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Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24688-1-AP targets PRY in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20232 Product name: Recombinant human PRY2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-63 aa of BC153128 Sequence: MGATGLGFLLSWRQDNLNGTDCQGCNILYFSETTGSMCSELSLNRGLEARRKKDLKDSFLWRY 相同性解析による交差性が予測される生物種 |
| Full Name | PTPN13-like, Y-linked 2 |
| Calculated molecular weight | 147 aa, 17 kDa |
| Observed molecular weight | 7 kDa |
| GenBank accession number | BC153128 |
| Gene Symbol | PRY |
| Gene ID (NCBI) | 442862 |
| RRID | AB_2879672 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14603 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRY is Testis-specific PTP-BL-related Y protein which has two isoforms with MW 16 and 6-8 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRY antibody 24688-1-AP | Download protocol |
| IHC protocol for PRY antibody 24688-1-AP | Download protocol |
| WB protocol for PRY antibody 24688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |