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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRUNE Polyclonal antibody (18537-1-AP)

    WB analysis using 18537-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18537-1-AP (PRUNE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PRUNE Polyclonal antibody (18537-1-AP)

    WB analysis of HepG2 using 18537-1-AP

    WB result of PRUNE antibody (18537-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRUNE transfected HepG2 cells.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PRUNE Polyclonal antibody (18537-1-AP)

    IP experiment of HepG2 using 18537-1-AP

    IP result of anti-PRUNE (IP:18537-1-AP, 3ug; Detection:18537-1-AP 1:500) with HepG2 cells lysate 1720ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRUNE Polyclonal antibody (18537-1-AP)

    IHC staining of human liver cancer using 18537-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRUNE Polyclonal antibody (18537-1-AP)

    IHC staining of human liver cancer using 18537-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRUNE Polyclonal antibody (18537-1-AP)

    IF Staining of HepG2 using 18537-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRUNE antibody (18537-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO Validated

PRUNE Polyclonal antibody

PRUNE Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18537-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Exopolyphosphatase PRUNE1, EC:3.6.1.1, Drosophila-related expressed sequence 17, DRES-17, DRES17

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18537-1-AP targets PRUNE in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13275

Product name: Recombinant human PRUNE protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 103-453 aa of BC025304

Sequence: ILVDHHILSKSDTALEEAVAEVLDHRPIEPKHCPPCHVSVELVGSCATLVTERILQGAPEILDRQTAALLHGTIILDCVNMDLKIGKATPKDSKYVEKLEALFPDLPKRNDIFDSLQKAKFDVSGLTTEQMLRKDQKTIYRQGVKVAISAIYMDLEAFLQRSNLLADLHAFCQAHSYDVLVAMTIFFNTHNEPVRQLAIFCPHVALQTTICEVLERSHSPPLKLTPASSTHPNLHAYLQGNTQVSRKKLLPLLQEALSAYFDSMKIPSGQPETADVSREQVDKELDRASNSLISGLSQDEEDPPLPPTPMNSLVDECPLDQGLPKLSAEAVFEKCSQISLSQSTTASLSKK

相同性解析による交差性が予測される生物種
Full Name prune homolog (Drosophila)
Calculated molecular weight 453 aa, 50 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC025304
Gene Symbol PRUNE
Gene ID (NCBI) 58497
RRIDAB_2172338
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TP1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PRUNE antibody 18537-1-APDownload protocol
IHC protocol for PRUNE antibody 18537-1-APDownload protocol
IP protocol for PRUNE antibody 18537-1-APDownload protocol
WB protocol for PRUNE antibody 18537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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