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Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using PRTN3 Polyclonal antibody (27153-1-AP)

    WB analysis of human spleen using 27153-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 27153-1-AP (PRTN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using PRTN3 Polyclonal antibody (27153-1-AP)

    WB analysis of human placenta using 27153-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27153-1-AP (PRTN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PRTN3 Polyclonal antibody (27153-1-AP)

    IHC staining of human spleen using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PRTN3 Polyclonal antibody (27153-1-AP)

    IHC staining of human spleen using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PRTN3 Polyclonal antibody (27153-1-AP)

    IHC staining of human tonsillitis using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PRTN3 Polyclonal antibody (27153-1-AP)

    IHC staining of human tonsillitis using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PRTN3 Polyclonal antibody (27153-1-AP)

    IHC staining of human tonsillitis using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PRTN3 Polyclonal antibody (27153-1-AP)

    IHC staining of human tonsillitis using 27153-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PR3/PRTN3 Polyclonal antibody

PR3/PRTN3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27153-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

PRTN3, PR3, NP-4, Myeloblastin, MBN

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman spleen tissue, human placenta tissue
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27153-1-AP targets PR3/PRTN3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25915

Product name: Recombinant human PRTN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 89-150 aa of BC096183

Sequence: NVRTQEPTQQHFSVAQVFLNNYDAENKLNDVLLIQLSSPANLSASVATVQLPQQDQPVPHGT

相同性解析による交差性が予測される生物種
Full Name proteinase 3
Calculated molecular weight 256 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC096183
Gene Symbol PRTN3
Gene ID (NCBI) 5657
RRIDAB_2880777
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP24158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PR3/PRTN3 antibody 27153-1-APDownload protocol
WB protocol for PR3/PRTN3 antibody 27153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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