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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRSS21 Polyclonal antibody (32522-1-AP)

    WB analysis using 32522-1-AP (same clone as 32522-1-PBS)

    Mouse testis lysates were subjected to SDS PAGE followed by western blot with 32522-1-AP (PRSS21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32522-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PRSS21 Polyclonal antibody (32522-1-AP)

    IHC staining of mouse testis using 32522-1-AP (same clone as 32522-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32522-1-AP (PRSS21 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32522-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PRSS21 Polyclonal antibody (32522-1-AP)

    IHC staining of mouse testis using 32522-1-AP (same clone as 32522-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32522-1-AP (PRSS21 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32522-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using PRSS21 Polyclonal antibody (32522-1-AP)

    FC experiment of A431 using 32522-1-AP (same clone as 32522-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Prss21 Polyclonal Antibody (32522-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 32522-1-PBS in a different storage buffer formulation.

PRSS21 Polyclonal antibody, PBS Only

PRSS21 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 32522-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

EC:3.4.21.-, Eosinophil serine protease 1, ESP1, ESP-1, Serine protease 21

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32522-1-PBS targets PRSS21 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag38156

Product name: Recombinant human PRSS21 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-314 aa of BC074999

Sequence: MGARGALLLALLLARAGLRKPESQEAAPLSGPCGRRVITSRIVGGEDAELGRWPWQGSLRLWDSHVCGVSLLSHRWALTAAHCFETYSDLSDPSGWMVQFGQLTSMPSFWSLQAYYTRYFVSNIYLSPRYLGNSPYDIALVKLSAPVTYTKHIQPICLQASTFEFENRTDCWVTGWGYIKEDEALPSPHTLQEVQVAIINNSMCNHLFLKYSFRKDIFGDMVCAGNAQGGKDACFGDSGGPLACNKNGLWYQIGVVSWGVGCGRPNRPGVYTNISHHFEWIQKLMAQSGMSQPDPSWPLLFFPLLWALPLLGPV

相同性解析による交差性が予測される生物種
Full Name protease, serine, 21 (testisin)
Calculated molecular weight 314 aa, 35 kDa
GenBank accession numberBC074999
Gene Symbol PRSS21
Gene ID (NCBI) 10942
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6M0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRSS21 (Serine Protease 21), a trypsin family member, may be active on peptide linkages involving the carboxyl group of lysine or arginine. In terms of disease association, PRSS21 is implicated in various cancers, such as gastric cancer, testicular cancer, and ovarian cancer.

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