Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11618-1-AP targets PRRG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2197 Product name: Recombinant human PRRG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-179 aa of BC026032 Sequence: MSLGPEVFLGPPEAQSFLSSHTRIPRANHWDLELLTPGNLERECLEERCSWEEAREYFEDNTLTERFWESYIYNGKGGRGRVDVASLAVGLTCGILLIVLAGLGAFWYLRWRQHRGQQPCPQEAGLISPLSPLNPLGPPTPLPPPPPPPPGLPTYEQALAASGVHDAPPPPYTSLRRPH 相同性解析による交差性が予測される生物種 |
| Full Name | proline rich Gla (G-carboxyglutamic acid) 2 |
| Calculated molecular weight | 179 aa, 20 kDa |
| Observed molecular weight | 16-22 kDa |
| GenBank accession number | BC026032 |
| Gene Symbol | PRRG2 |
| Gene ID (NCBI) | 5639 |
| RRID | AB_2171684 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14669 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proline-rich Gla protein 2 (PRRG2) represents one of the four known transmembrane-carboxyglutamic acid proteins in vertebrates. As a founding member of the vitamin K-dependent, single-pass integral membrane protein family, PRRG2 is characterized by an extracellular N-terminus consisting of approximately 45 amino acids rich in Gla, as well as a conserved Pro/Leu-Pro-xaa-Tyr (PY) motif proximal to its C-terminus. PRRG2 primarily localizes to the cell surface, where the Gla domain is exposed extracellularly, serving as a pivotal gene node with positive autoregulatory function that plays a critical role in maintaining human hemostasis (PMID: 38227081). The calculated molecular weight of PRRG2 is usually approximately 22 kda.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRRG2 antibody 11618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
