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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRRC1 Polyclonal antibody

PRRC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27887-1-AP

Synonyms

proline rich coiled coil 1, Protein PRRC1, PRRC1

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 27887-1-AP

    WB analysis of HeLa using 27887-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27887-1-AP (PRRC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 27887-1-AP

    WB analysis of mouse heart using 27887-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 27887-1-AP (PRRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 27887-1-AP

    IHC staining of human pancreas using 27887-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27887-1-AP (PRRC1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 27887-1-AP

    IHC staining of human pancreas using 27887-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27887-1-AP (PRRC1 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27887-1-AP targets PRRC1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRRC1 fusion protein Ag27349 相同性解析による交差性が予測される生物種
Full Name proline-rich coiled-coil 1
Calculated molecular weight 445 aa, 47 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC017066
Gene symbol PRRC1
Gene ID (NCBI) 133619
RRIDAB_2881005
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRRC1 antibody 27887-1-APDownload protocol
IHC protocol for PRRC1 antibody 27887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols