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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRR14 Polyclonal antibody (22819-1-AP)

    WB analysis using 22819-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRR14 Polyclonal antibody (22819-1-AP)

    WB analysis using 22819-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using PRR14 Polyclonal antibody (22819-1-AP)

    WB analysis of HL-60 using 22819-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PRR14 Polyclonal antibody (22819-1-AP)

    WB analysis of mouse brain using 22819-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PRR14 Polyclonal antibody (22819-1-AP)

    IHC staining of human stomach using 22819-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 22819-1-AP (PRR14 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using PRR14 Polyclonal antibody (22819-1-AP)

    IHC staining of human stomach using 22819-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 22819-1-AP (PRR14 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

PRR14 Polyclonal antibody

PRR14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22819-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Proline-rich protein 14, Proline rich protein 14, proline rich 14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, HL-60 cells, MCF-7 cells, SW480 cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22819-1-AP targets PRR14 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRR14 fusion protein Ag14119 相同性解析による交差性が予測される生物種
Full Name proline rich 14
Calculated molecular weight 585 aa, 64 kDa
Observed molecular weight 64-70 kDa
GenBank accession numberBC000119
Gene Symbol PRR14
Gene ID (NCBI) 78994
RRIDAB_2879174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRR14 (proline-rich protein 14) is a 585-amino acid protein with a calculated molecular weight of 64 kDa. This protein functions to tether heterochromatin to the nuclear periphery during interphase, through associations with heterochromatin protein 1 (HP1) and the nuclear lamina. After disassembly early in mitosis, PRR14 reassembles in two steps, first binding to anaphase chromosomes through HP1, followed by association with the nuclear lamina in telophase. PRR14 may thereby play a role in specifying HP1-bound heterochromatin for reattachment to the nuclear lamina at mitotic exit. (PMID: 24209742; 24637393)

Protocols

Product Specific Protocols
WB protocol for PRR14 antibody 22819-1-APDownload protocol
IHC protocol for PRR14 antibody 22819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nucleus

PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina

Authors - Anna A Kiseleva
  • KD Validated
View Article