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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRR11 Polyclonal antibody (27422-1-AP)

    WB analysis using 27422-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27422-1-AP (PRR11 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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PRR11 Polyclonal antibody

PRR11 Polyclonal Antibody for WB, ELISA
Cat No. 27422-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Proline rich protein 11, proline rich 11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, L02 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27422-1-AP targets PRR11 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRR11 fusion protein Ag26636 相同性解析による交差性が予測される生物種
Full Name proline rich 11
Calculated molecular weight 360 aa, 40 kDa
Observed molecular weight38 kDa
GenBank accession numberBC008669
Gene Symbol PRR11
Gene ID (NCBI) 55771
RRIDAB_3669600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HE9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRR11, or Proline-rich protein 11, is a protein-coding gene situated on chromosome 17q22-23, which plays a significant role in various cellular processes. The protein itself is composed of a nuclear localization signal (NLS), two proline-rich regions (PRs), and a zinc finger domain (ZFD) (PMID: 36551227). PRR11 has been implicated in the regulation of cell cycle, and it is involved in multiple aspects of cancer biology, including proliferation, migration, invasion, cell-cycle progression, apoptosis, autophagy, and chemotherapy resistance. It serves as a prognostic indicator in certain human cancers and is considered a potential target for therapeutic interventions (PMID: 36739300).

Protocols

Product Specific Protocols
WB protocol for PRR11 antibody 27422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols