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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRPH2 Recombinant monoclonal antibody (85043-1-RR)

    WB analysis using 85043-1-RR (same clone as 85043-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85043-1-RR (PRPH2 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRPH2 Recombinant monoclonal antibody (85043-1-RR)

    IHC staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRPH2 Recombinant monoclonal antibody (85043-1-RR)

    IHC staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRPH2 Recombinant monoclonal antibody (85043-1-RR)

    IF Staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRPH2 Recombinant monoclonal antibody (85043-1-RR)

    IF Staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

PRPH2 Recombinant monoclonal antibody, PBS Only

PRPH2 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85043-1-PBS
Clone No.242170F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

242170F4, AOFMD, AVMD, Peripherin 2, Peripherin2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85043-1-PBS targets PRPH2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag12555

Product name: Recombinant human PRPH2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 124-346 aa of BC074720

Sequence: GSLENTLGQGLKNGMKYYRDTDTPGRCFMKKTIDMLQIEFKCCGNNGFRDWFEIQWISNRYLDFSSKEVKDRIKSNVDGRYLVDGVPFSCCNPSSPRPCIQYQITNNSAHYSYDHQTEELNLWVRGCRAALLSYYSSLMNSMGVVTLLIWLFEVTITIGLRYLQTSLDGVSNPEESESESEGWLLEKSVPETWKAFLESVKKLGKGNQVEAEGAGAGQAPEAG

相同性解析による交差性が予測される生物種
Full Name peripherin 2 (retinal degeneration, slow)
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight30-40 kDa
GenBank accession numberBC074720
Gene Symbol PRPH2
Gene ID (NCBI) 5961
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP23942
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in the stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).

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