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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PRPF8 Polyclonal antibody (11171-1-AP)

    WB analysis of Jurkat using 11171-1-AP (same clone as 11171-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using PRPF8 Polyclonal antibody (11171-1-AP)

    WB analysis of human placenta using 11171-1-AP (same clone as 11171-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PRPF8 Polyclonal antibody (11171-1-AP)

    WB analysis of K-562 using 11171-1-AP (same clone as 11171-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PRPF8 Polyclonal antibody (11171-1-AP)

    WB analysis of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PRPF8 Polyclonal antibody (11171-1-AP)

    IP experiment of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

    IP result of anti-PRPF8 (IP:11171-1-AP, 4ug; Detection:11171-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRPF8 Polyclonal antibody (11171-1-AP)

    IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRPF8 Polyclonal antibody (11171-1-AP)

    IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PRPF8 Polyclonal antibody (11171-1-AP)

    IHC staining of human stomach cancer using 11171-1-AP (same clone as 11171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

PRPF8 Polyclonal antibody, PBS Only

PRPF8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11171-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11171-1-PBS targets PRPF8 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1652

Product name: Recombinant human PRPF8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2037-2335 aa of BC064370

Sequence: SQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

相同性解析による交差性が予測される生物種
Full Name PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
Calculated molecular weight 274 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC064370
Gene Symbol PRPF8
Gene ID (NCBI) 10594
RRIDAB_2171179
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P2Q9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.

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