Validation Data Gallery
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Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, U-251 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86123-1-RR targets PRPF6 in applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PRPF6 fusion protein Ag13817 相同性解析による交差性が予測される生物種 |
Full Name | PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) |
Calculated molecular weight | 941 aa, 107 kDa |
Observed molecular weight | 100-108 kDa |
GenBank accession number | BC001666 |
Gene Symbol | PRPF6 |
Gene ID (NCBI) | 24148 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O94906 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRPF6 is involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, it can enhance dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.
Protocols
Product Specific Protocols | |
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WB protocol for PRPF6 antibody 86123-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |