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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PRPF6 Polyclonal antibody (23929-1-AP)

    WB analysis of HEK-293 using 23929-1-AP (same clone as 23929-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PRPF6 Polyclonal antibody (23929-1-AP)

    WB analysis of HEK-293 using 23929-1-AP (same clone as 23929-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PRPF6 Polyclonal antibody (23929-1-AP)

    WB analysis of NIH/3T3 using 23929-1-AP (same clone as 23929-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PRPF6 Polyclonal antibody (23929-1-AP)

    WB analysis of HeLa using 23929-1-AP (same clone as 23929-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PRPF6 Polyclonal antibody (23929-1-AP)

    WB analysis of NIH/3T3 using 23929-1-AP (same clone as 23929-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PRPF6 Polyclonal antibody (23929-1-AP)

    WB analysis of HeLa using 23929-1-AP (same clone as 23929-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PRPF6 Polyclonal antibody (23929-1-AP)

    IP experiment of HeLa using 23929-1-AP (same clone as 23929-1-PBS)

    IP result of anti-PRPF6 (IP:23929-1-AP, 4ug; Detection:23929-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF6 Polyclonal antibody (23929-1-AP)

    IHC staining of human testis using 23929-1-AP (same clone as 23929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF6 Polyclonal antibody (23929-1-AP)

    IHC staining of human testis using 23929-1-AP (same clone as 23929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PRPF6 Polyclonal antibody, PBS Only

PRPF6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 23929-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23929-1-PBS targets PRPF6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13817

Product name: Recombinant human PRPF6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 592-941 aa of BC001666

Sequence: SLEALLQRAVAHCPKAEVLWLMGAKSKWLAGDVPAARSILALAFQANPNSEEIWLAAVKLESENDEYERARRLLAKARSSAPTARVFMKSVKLEWVQDNIRAAQDLCEEALRHYEDFPKLWMMKGQIEEQKEMMEKAREAYNQGLKKCPHSTPLWLLLSRLEEKIGQLTRARAILEKSRLKNPKNPGLWLESVRLEYRAGLKNIANTLMAKALQECPNSGILWSEAIFLEARPQRRTKSVDALKKCEHDPHVLLAVAKLFWSQRKITKAREWFHRTVKIDSDLGDAWAFFYKFELQHGTEEQQEEVRKRCESAEPRHGELWCAVSKDIANWQKKIGDILRLVAGRIKNTF

相同性解析による交差性が予測される生物種
Full Name PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)
Calculated molecular weight 941 aa, 107 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC001666
Gene Symbol PRPF6
Gene ID (NCBI) 24148
RRIDAB_2879365
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDO94906
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRPF6 is involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, it can enhance dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.

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