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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PRPF40A Polyclonal antibody (17392-1-AP)

    WB analysis of HEK-293 using 17392-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRPF40A Polyclonal antibody (17392-1-AP)

    WB analysis of HeLa using 17392-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRPF40A Polyclonal antibody (17392-1-AP)

    WB analysis of HeLa using 17392-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PRPF40A Polyclonal antibody (17392-1-AP)

    WB analysis of mouse brain using 17392-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PRPF40A Polyclonal antibody (17392-1-AP)

    IP experiment of HEK-293 using 17392-1-AP

    IP result of anti-PRPF40A (IP:17392-1-AP, 4ug; Detection:17392-1-AP 1:500) with HEK-293 cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PRPF40A Polyclonal antibody (17392-1-AP)

    IHC staining of human lung cancer using 17392-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17392-1-AP (PRPF40A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PRPF40A Polyclonal antibody (17392-1-AP)

    IHC staining of human lung cancer using 17392-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17392-1-AP (PRPF40A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRPF40A Polyclonal antibody

PRPF40A Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17392-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

Fas ligand associated factor 1, Fas ligand-associated factor 1, FBP 11, FBP11, FLAF1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17392-1-AP targets PRPF40A in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11290

Product name: Recombinant human PRPF40A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 743-957 aa of BC027178

Sequence: MKRKESAFKSMLKQAAPPIELDAVWEDIRERFVKEPAFEDITLESERKRIFKDFMHVLEHECQHHHSKNKKHSKKSKKHHRKRSRSRSGSDSDDDDSHSKKKRQRSESRSASEHSSSAESERSYKKSKKHKKKSKKRRHKSDSPESDAEREKDKKEKDRESEKDRTRQRSESKHKSPKKKTGKDSGNWDTSGSELSEGELEKRRRTLLEQLDDDQ

相同性解析による交差性が予測される生物種
Full Name PRP40 pre-mRNA processing factor 40 homolog A (S. cerevisiae)
Calculated molecular weight 957 aa, 109 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC027178
Gene Symbol PRPF40A
Gene ID (NCBI) 55660
RRIDAB_2171031
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75400
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PRPF40A antibody 17392-1-APDownload protocol
IP protocol for PRPF40A antibody 17392-1-APDownload protocol
WB protocol for PRPF40A antibody 17392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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