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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PRPF39 Polyclonal antibody (24480-1-AP)

    WB analysis of K-562 using 24480-1-AP (same clone as 24480-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24480-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using PRPF39 Polyclonal antibody (24480-1-AP)

    WB analysis of Raji using 24480-1-AP (same clone as 24480-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24480-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PRPF39 Polyclonal antibody (24480-1-AP)

    WB analysis of COLO 320 using 24480-1-AP (same clone as 24480-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24480-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP (same clone as 24480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 24480-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP (same clone as 24480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 24480-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP (same clone as 24480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 24480-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP (same clone as 24480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24480-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP (same clone as 24480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24480-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP (same clone as 24480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 24480-1-PBS in a different storage buffer formulation.

PRPF39 Polyclonal antibody, PBS Only

PRPF39 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24480-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24480-1-PBS targets PRPF39 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21683

Product name: Recombinant human PRPF39 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-348 aa of BC125126

Sequence: MEQSPDDSPNVNASTEETEMASAVDLPVTLTETEANFPPEYEKFWKTVENNPQDFTGWVYLLQYVEQENHLMAARKAFDRFFIHYPYCYGYWKKYADLEKRHDNIKPSDEVYRRGLQAIPLSVDLWIHYINFLKETLDPGDPETNNTIRGTFEHAVLAAGTDFRSDRLWEMYINWENEQGNLREVTAIYDRILGIPTQLYSHHFQRFKEHVQNNLPRDLLTGEQFIQLRRELASVNGHSGDDGPPGDDLPSGIEDITDPAKLITEIENMRHRIIEIHQEMFNYNEHEVSKRWTFEEGIKRPYFHVKPLEKAQLKNWKEYLEFEIENGTHERVVVLFERCVISCALYEE

相同性解析による交差性が予測される生物種
Full Name PRP39 pre-mRNA processing factor 39 homolog (S. cerevisiae)
Calculated molecular weight 669 aa, 78 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC125126
Gene Symbol PRPF39
Gene ID (NCBI) 55015
RRIDAB_2879563
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UA1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRPF39 also termed as Pre mRNA processing factor 39 or PRP39 homolog is a 669 amino acid protein, which contains 7 HAT repeats and belongs to the PRP39 family. PRPF39 localizes in nucleus and may play a role in mRNA splicing.

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