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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRPF31 Recombinant antibody (86315-2-RR)

    WB analysis using 86315-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86315-2-RR (PRPF31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PRPF31 Recombinant antibody (86315-2-RR)

    IF Staining of Jurkat using 86315-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PRPF31 antibody (86315-2-RR, Clone: 251005H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRPF31 Recombinant antibody (86315-2-RR)

    IF Staining of HeLa using 86315-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRPF31 antibody (86315-2-RR, Clone: 251005H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86315-2-RR

    Affinity and Kinetic Characterization of 86315-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86315-2-RR against Human PRPF31 were performed. The affinity constant is below 1 pM.

PRPF31 Recombinant monoclonal antibody

PRPF31 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86315-2-RR
Clone No.251005H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

hPrp31, NY BR 99, Protein 61K, PRP31, RP11

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, A549 cells, Caco-2 cells, OVCAR-3 cells
Positive IF/ICC detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86315-2-RR targets PRPF31 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26716

Product name: Recombinant human PRPF31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-212 aa of NM_015629

Sequence: MVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRM

相同性解析による交差性が予測される生物種
Full Name PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
Calculated molecular weight 55 kDa
Observed molecular weight55-60 kDa
GenBank accession numberNM_015629
Gene Symbol PRPF31
Gene ID (NCBI) 26121
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8WWY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.

Protocols

Product Specific Protocols
IF protocol for PRPF31 antibody 86315-2-RRDownload protocol
WB protocol for PRPF31 antibody 86315-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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