Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, Caco-2 cells, OVCAR-3 cells, NIH/3T3 cells, 3T3-L1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86315-1-RR targets PRPF31 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag26716 Product name: Recombinant human PRPF31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 101-212 aa of NM_015629 Sequence: MVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRM 相同性解析による交差性が予測される生物種 |
| Full Name | PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | NM_015629 |
| Gene Symbol | PRPF31 |
| Gene ID (NCBI) | 26121 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8WWY3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.