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Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using PRPF3 Polyclonal antibody (10106-1-AP)

    WB analysis of Y79 using 10106-1-AP (same clone as 10106-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PRPF3 Polyclonal antibody (10106-1-AP)

    WB analysis of HepG2 using 10106-1-AP (same clone as 10106-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using PRPF3 Polyclonal antibody (10106-1-AP)

    WB analysis of L02 using 10106-1-AP (same clone as 10106-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using PRPF3 Polyclonal antibody (10106-1-AP)

    WB analysis of mouse liver using 10106-1-AP (same clone as 10106-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using PRPF3 Polyclonal antibody (10106-1-AP)

    WB analysis of mouse liver using 10106-1-AP (same clone as 10106-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PRPF3 Polyclonal antibody (10106-1-AP)

    WB analysis of mouse brain using 10106-1-AP (same clone as 10106-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using PRPF3 Polyclonal antibody (10106-1-AP)

    IP experiment of mouse liver using 10106-1-AP (same clone as 10106-1-PBS)

    IP result of anti-PRPF3 (IP:10106-1-AP, 5ug; Detection:10106-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PRPF3 Polyclonal antibody (10106-1-AP)

    IHC staining of human stomach using 10106-1-AP (same clone as 10106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10106-1-AP (PRPF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

PRPF3 Polyclonal antibody, PBS Only

PRPF3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10106-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

RP18, Prp3p, PRP3, Pre-mRNA-splicing factor 3, HPRP3P

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10106-1-PBS targets PRPF3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0154

Product name: Recombinant human PRPF3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 229-493 aa of BC000184

Sequence: MVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEIELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLMPPPEPKVRISNLMRVLGTEAVQDP

相同性解析による交差性が予測される生物種
Full Name PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae)
Calculated molecular weight 78 kDa
Observed molecular weight 90 kDa, 78 kDa
GenBank accession numberBC000184
Gene Symbol PRPF3
Gene ID (NCBI) 9129
RRIDAB_2170994
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43395
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3. A predicted molecular mass of 77 kDa and the gene is named PRPF3 because it is homologous to the yeast PRP3 gene. We noticed that although the predicted molecular mass of PRPF3 is77 kDa, the protein produced in E.coli migrated like a 90 kDa protein in SDS-PAGE. (PMID: 9328476)

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