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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRPF19 Recombinant monoclonal antibody (88331-1-RR)

    WB analysis using 88331-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88331-1-RR (PRPF19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRPF19 Recombinant monoclonal antibody (88331-1-RR)

    IF Staining of HeLa using 88331-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRPF19 antibody (88331-1-RR, Clone: 260837A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PRPF19 Recombinant monoclonal antibody

PRPF19 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 88331-1-RR
Clone No.260837A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.3.2.27, hPso4, Pre-mRNA-processing factor 19, PRP19, PSO4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, PC-3 cells, Jurkat cells, A431 cells, mouse kidnet tissue, rat kidney tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88331-1-RR targets PRPF19 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7700

Product name: Recombinant human PRPF19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 155-504 aa of BC008719

Sequence: GAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL

相同性解析による交差性が予測される生物種
Full Name PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
Calculated molecular weight 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC008719
Gene Symbol PRPF19
Gene ID (NCBI) 27339
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UMS4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.

Protocols

Product Specific Protocols
IF protocol for PRPF19 antibody 88331-1-RRDownload protocol
WB protocol for PRPF19 antibody 88331-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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