Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
10261-1-AP targets PRPF18 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0329 Product name: Recombinant human PRPF18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-47 aa of BC002572 Sequence: MDILKSEILRKRQLVEDRNLLREYVKANDAYLQMAIGNAPWPIGVTM 相同性解析による交差性が予測される生物種 |
| Full Name | PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) |
| Calculated molecular weight | 5 aa, 475 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC002572 |
| Gene Symbol | PRPF18 |
| Gene ID (NCBI) | 8559 |
| RRID | AB_3741814 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99633 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRPF18 antibody 10261-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |