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Validation Data Gallery

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  • Western Blot (WB) analysis of HuH-7 cells using PROX1 Monoclonal antibody (67438-1-Ig)

    WB analysis of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using PROX1 Monoclonal antibody (67438-1-Ig)

    WB analysis of rat liver using 67438-1-Ig (same clone as 67438-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PROX1 Monoclonal antibody (67438-1-Ig)

    IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PROX1 Monoclonal antibody (67438-1-Ig)

    IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using PROX1 Monoclonal antibody (67438-1-Ig)

    IF Staining of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (67438-1-Ig, Clone: 1D4B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

PROX1 Monoclonal antibody, PBS Only

PROX1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67438-1-PBS
Clone No.1D4B1

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1D4B1, Homeobox prospero-like protein PROX1, prospero homeobox 1, Prospero homeobox protein 1, PROX-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67438-1-PBS targets PROX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9681

Product name: Recombinant human PROX1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 402-737 aa of BC024201

Sequence: FHTANQRLQCFGDVIIPNPLDTFGNVQMASSTDQTEALPLVVRKNSSDQSASGPAAGGHHQPLHQSPLSATTGFTTSTFRHPFPLPLMAYPFQSPLGAPSGSFSGKDRASPESLDLTRDTTSLRTKMSSHHLSHHPCSPAHPPSTAEGLSLSLIKSECGDLQDMSEISPYSGSAMQEGLSPNHLKKAKLMFFYTRYPSSNMLKTYFSDVKFNRCITSQLIKWFSNFREFYYIQMEKYARQAINDGVTSTEELSITRDCELYRALNMHYNKANDFEVPERFLEVAQITLREFFNAIIAGKDVDPSWKKAIYKVICKLDSEVPEIFKSPNCLQELLHE

相同性解析による交差性が予測される生物種
Full Name prospero homeobox 1
Calculated molecular weight 737 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC024201
Gene Symbol PROX1
Gene ID (NCBI) 5629
RRIDAB_2882674
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ92786
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.

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