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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using PROS1 Polyclonal antibody (16910-1-AP)

    WB analysis of human placenta using 16910-1-AP (same clone as 16910-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PROS1 Polyclonal antibody (16910-1-AP)

    WB analysis using 16910-1-AP (same clone as 16910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using PROS1 Polyclonal antibody (16910-1-AP)

    WB analysis of human placenta using 16910-1-AP (same clone as 16910-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse placenta tissue using PROS1 Polyclonal antibody (16910-1-AP)

    WB analysis of mouse placenta using 16910-1-AP (same clone as 16910-1-PBS)

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PROS1 Polyclonal antibody (16910-1-AP)

    IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PROS1 Polyclonal antibody (16910-1-AP)

    IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PROS1 Polyclonal antibody (16910-1-AP)

    IF Staining of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PROS1 antibody (16910-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using PROS1 Polyclonal antibody (16910-1-AP)

    FC experiment of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PROS1 (16910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

PROS1 Polyclonal antibody, PBS Only

PROS1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 16910-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

PROS, Vitamin K-dependent protein S

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16910-1-PBS targets PROS1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10539

Product name: Recombinant human PROS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 328-676 aa of BC015801

Sequence: DFRTYDSEGVILYAESIDHSAWLLIALRGGKIEVQLKNEHTSKITTGGDVINNGLWNMVSVEELEHSISIKIAKEAVMDINKPGPLFKPENGLLETKVYFAGFPRKVESELIKPINPRLDGCIRSWNLMKQGASGIKEIIQEKQNKHCLVTVEKGSYYPGSGIAQFHIDYNNVSSAEGWHVNVTLNIRPSTGTGVMLALVSGNNTVPFAVSLVDSTSEKSQDILLSVENTVIYRIQALSLCSDQQSHLEFRVNRNNLELSTPLKIETISHEDLQRQLAVLDKAMKAKVATYLGGLPDVPFSATPVNAFYNGCMEVNINGVQLDLDEAISKHNDIRAHSCPSVWKKTKNS

相同性解析による交差性が予測される生物種
Full Name protein S (alpha)
Calculated molecular weight 75 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC015801
Gene Symbol PROS1
Gene ID (NCBI) 5627
RRIDAB_2300207
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07225
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.

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