Validation Data Gallery
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Tested Applications
| Positive WB detected in | human placenta tissue, mouse embryo |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18739-1-AP targets PROP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | PROP paired-like homeobox 1 |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC069076 |
| Gene Symbol | PROP1 |
| Gene ID (NCBI) | 5626 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75360 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PROP1 belongs to the paired homeobox family. It contains one homeobox DNA-binding domain. PROP1 is possibly involved in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes. It is expressed specifically in embryonic pituitary. Mutation of PROP1 will cause combined pituitary hormone deficiency (CPHD) which is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PROP1 antibody 18739-1-AP | Download protocol |
| WB protocol for PROP1 antibody 18739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |