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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRODH Polyclonal antibody (22980-1-AP)

    WB analysis using 22980-1-AP (same clone as 22980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22980-1-AP (PRODH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRODH Polyclonal antibody (22980-1-AP)

    WB analysis using 22980-1-AP (same clone as 22980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22980-1-AP (PRODH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRODH Polyclonal antibody (22980-1-AP)

    WB analysis using 22980-1-AP (same clone as 22980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22980-1-AP (PRODH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human cerebellum using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human cerebellum using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human liver cancer using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human liver cancer using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human liver cancer using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human liver cancer using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human breast cancer using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human breast cancer using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human skeletal muscle using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PRODH Polyclonal antibody (22980-1-AP)

    IHC staining of human skeletal muscle using 22980-1-AP (same clone as 22980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 22980-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PRODH Polyclonal antibody, PBS Only

PRODH Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22980-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22980-1-PBS targets PRODH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18429

Product name: Recombinant human PRODH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 143-492 aa of BC118597

Sequence: LAKWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAYDNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAKAKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPYLSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA

相同性解析による交差性が予測される生物種
Full Name proline dehydrogenase (oxidase) 1
Calculated molecular weight 600 aa, 68 kDa
Observed molecular weight 56 kDa, 66 kDa
GenBank accession numberBC118597
Gene Symbol PRODH
Gene ID (NCBI) 5625
RRIDAB_2879191
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43272
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRODH(Proline dehydrogenase 1, mitochondrial) is also named as PIG6, HSPOX2, PRODH1, PRODH2, POX, SCZD4, TP53I6 and belongs to the proline oxidase family. It is an oxidoreductase involved in the transfer of redox potential across the mitochondrial membrane and catalyzes the rate-limiting oxidation of proline to pyrroline- 5-carboxylate (P5C). High PRODH activity is sufficient to induce mitochondria-mediated apoptosis in the presence of proline. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1) and defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). This protein has 3 isoforms produced by alternative splicing with the molecular mass of 68 kDa, 59 kDa and 56 kDa.

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