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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPCR/CD201 Polyclonal antibody (32134-1-AP)

    WB analysis using 32134-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EPCR/CD201 Polyclonal antibody (32134-1-AP)

    WB analysis using 32134-1-AP

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPCR/CD201 Polyclonal antibody (32134-1-AP)

    IHC staining of human placenta using 32134-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPCR/CD201 Polyclonal antibody (32134-1-AP)

    IHC staining of human placenta using 32134-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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EPCR/CD201 Polyclonal antibody

EPCR/CD201 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32134-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Activated protein C receptor, APC receptor, CD201, Centrocyclin, Centrosomal protein CCD41

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, bEnd.3 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32134-1-AP targets EPCR/CD201 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name protein C receptor, endothelial
Calculated molecular weight27kDa
Observed molecular weight 27 kDa, 40-50 kDa
GenBank accession numberNM_011171.2
Gene Symbol PROCR
Gene ID (NCBI) 19124
RRIDAB_3670204
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ64695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective effects on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424). EPCR is approximately 46 kDa, truncated EPCR is 23 kDa, truncated EPCR with the transmembrane domain is 26 kDa, and soluble EPCR is 43 kDa (PMID: 11686350).

Protocols

Product Specific Protocols
WB protocol for EPCR/CD201 antibody 32134-1-APDownload protocol
IHC protocol for EPCR/CD201 antibody 32134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols