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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using PROC Polyclonal antibody (25382-1-AP)

    WB analysis of mouse liver using 25382-1-AP (same clone as 25382-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25382-1-AP (PROC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PROC Polyclonal antibody (25382-1-AP)

    IHC staining of human liver using 25382-1-AP (same clone as 25382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PROC Polyclonal antibody (25382-1-AP)

    IHC staining of human liver using 25382-1-AP (same clone as 25382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25382-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PROC Polyclonal antibody (25382-1-AP)

    IF Staining of HeLa using 25382-1-AP (same clone as 25382-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PROC antibody (25382-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25382-1-PBS in a different storage buffer formulation.

PROC Polyclonal antibody, PBS Only

PROC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25382-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Vitamin K-dependent protein C, Vitamin K dependent protein C, EC:3.4.21.69, Blood coagulation factor XIV, Autoprothrombin IIA

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25382-1-PBS targets PROC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11328

Product name: Recombinant human PROC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 113-461 aa of BC034377

Sequence: DGIGSFSCDCRSGWEGRFCQREVSFLNCSLDNGGCTHYCLEEVGWRRCSCAPGYKLGDDLLQCHPAVKFPCGRPWKRMEKKRSHLKRDTEDQEDQVDPRLIDGKMTRRGDSPWQVVLLDSKKKLACGAVLIHPSWVLTAAHCMDESKKLLVRLGEYDLRRWEKWELDLDIKEVFVHPNYSKSTTDNDIALLHLAQPATLSQTIVPICLPDSGLAERELNQAGQETLVTGWGYHSSREKEAKRNRTFVLNFIKIPVVPHNECSEVMSNMVSENMLCAGILGDRQDACEGDSGGPMVASFHGTWFLVGLVSWGEGCGLLHNYGVYTKVSRYLDWIHGHIRDKEAPQKSWAP

相同性解析による交差性が予測される生物種
Full Name protein C (inactivator of coagulation factors Va and VIIIa)
Calculated molecular weight 461 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC034377
Gene Symbol PROC
Gene ID (NCBI) 5624
RRIDAB_2880052
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP04070
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PROC belongs to the peptidase S1 family. PROC is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PMID:25618265). It exerts a protective effect on the endothelial cell barrier function (PMID:25651845).

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