Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 15395-1-AP (same clone as 15395-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 15395-1-AP (same clone as 15395-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 15395-1-AP (same clone as 15395-1-PBS)
IP result of anti-PRMT6 (IP:15395-1-AP, 4ug; Detection:15395-1-AP 1:1500) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15395-1-AP (same clone as 15395-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15395-1-AP (same clone as 15395-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 15395-1-AP (same clone as 15395-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 15395-1-AP (same clone as 15395-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15395-1-AP (same clone as 15395-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 15395-1-AP (same clone as 15395-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 15395-1-AP (same clone as 15395-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PRMT6 antibody (15395-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
FC experiment of MCF-7 using 15395-1-AP (same clone as 15395-1-PBS)
1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug PRMT6 Polyclonal antibody (15395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.
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1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug PRMT6 Polyclonal antibody (15395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PRMT6 Polyclonal antibody, PBS Only

PRMT6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 15395-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15395-1-PBS targets PRMT6 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7592 Product name: Recombinant human PRMT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC002729 Sequence: MIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGLEDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQMLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEAGVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKPLVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSGEITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAMED 相同性解析による交差性が予測される生物種
Full Name	protein arginine methyltransferase 6
Calculated molecular weight	42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC002729
Gene Symbol	PRMT6
Gene ID (NCBI)	55170
RRID	AB_2237723
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96LA8
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

PRMT6 belongs to the protein arginine methyltransferase (PRMT) family that catalyzes the mono- and dimethylation of arginine residues in a variety of proteins. PRMT6 is a nuclear protein and is predominantly expressed in testis and kidney. Western blot analysis using this antibody detected a major band around 42 kDa in HeLa cells.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15395-1-AP (same clone as 15395-1-PBS)

WB analysis of mouse brain using 15395-1-AP (same clone as 15395-1-PBS)

IP experiment of HEK-293 using 15395-1-AP (same clone as 15395-1-PBS)

IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)

IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)

IHC staining of human kidney using 15395-1-AP (same clone as 15395-1-PBS)