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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT5 Recombinant antibody (82750-2-RR)

    WB analysis using 82750-2-RR (same clone as 82750-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82750-2-RR (PRMT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PRMT5 Recombinant antibody (82750-2-RR)

    IP experiment of mouse brain using 82750-2-RR (same clone as 82750-2-PBS)

    IP result of anti-PRMT5 (IP:82750-2-RR, 4ug; Detection:82750-2-RR 1:3000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRMT5 Recombinant antibody (82750-2-RR)

    IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-2-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRMT5 Recombinant antibody (82750-2-RR)

    IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-2-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRMT5 Recombinant antibody (82750-2-RR)

    IF Staining of HeLa using 82750-2-RR (same clone as 82750-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRMT5 antibody (82750-2-RR, Clone: 250553B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82750-2-RR

    Affinity and Kinetic Characterization of 82750-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82750-2-RR against Human PRMT5 were performed. The affinity constant is 0.923 nM.

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PRMT5 Recombinant antibody, PBS Only

PRMT5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82750-2-PBS
Clone No.250553B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82750-2-PBS targets PRMT5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRMT5 fusion protein Ag9736 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 5
Calculated molecular weight 637 aa, 73 kDa
Observed molecular weight68 kDa
GenBank accession numberBC025979
Gene Symbol PRMT5
Gene ID (NCBI) 10419
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14744
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.