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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT3 Polyclonal antibody (17628-1-AP)

    WB analysis using 17628-1-AP (same clone as 17628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17628-1-AP (PRMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PRMT3 Polyclonal antibody (17628-1-AP)

    IHC staining of mouse liver using 17628-1-AP (same clone as 17628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17628-1-AP (PRMT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PRMT3 Polyclonal antibody (17628-1-AP)

    IHC staining of mouse liver using 17628-1-AP (same clone as 17628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17628-1-AP (PRMT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17628-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRMT3 Polyclonal antibody (17628-1-AP)

    IF Staining of HeLa using 17628-1-AP (same clone as 17628-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRMT3 antibody (17628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17628-1-PBS in a different storage buffer formulation.

PRMT3 Polyclonal antibody, PBS Only

PRMT3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17628-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17628-1-PBS targets PRMT3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11877

Product name: Recombinant human PRMT3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 178-531 aa of BC037544

Sequence: RAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLNKLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAKNKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMCTAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEALKGKVTVHKNKKDPRSLTVTLTLNNSTQTYGLQ

相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 3
Calculated molecular weight 531 aa, 60 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC037544
Gene Symbol PRMT3
Gene ID (NCBI) 10196
RRIDAB_2171654
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60678
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.

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