Validation Data Gallery
Tested Applications
| Positive WB detected in | SKOV-3 cells, HeLa cells, rat heart tissue, LNCaP cells, Jurkat cells, 4T1 cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
66885-1-Ig targets PRMT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1806 Product name: Recombinant human PRMT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 134-433 aa of BC000727 Sequence: ESLTDKVILDVGCGTGIISLFCAHYARPRAVYAVEASEMAQHTGQLVLQNGFADIITVYQQKVEDVVLPEKVDVLVSEWMGTCLLFEFMIESILYARDAWLKEDGVIWPTMAALHLVPCSADKDYRSKVLFWDNAYEFNLSALKSLAVKEFFSKPKYNHILKPEDCLSEPCTILQLDMRTVQISDLETLRGELRFDIRKAGTLHGFTAWFSVHFQSLQEGQPPQVLSTGPFHPTTHWKQTLFMMDDPVPVHTGDVVTGSVVLQRNPVWIRHMSVALSWAVTSRQDPTSQKVGEKVFPIWR 相同性解析による交差性が予測される生物種 |
| Full Name | protein arginine methyltransferase 2 |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC000727 |
| Gene Symbol | PRMT2 |
| Gene ID (NCBI) | 3275 |
| RRID | AB_2882217 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P55345 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRMT2 (also known as HRMT1L1) is a member of the arginine methyltransferase family which catalyzes arginine methylation and regulates diverse cellular processes, including transcription, translation, DNA repair, and RNA processing. Recently PRMT2 has been identified as a pro-tumorigenic factor in malignant gliomas.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRMT2 antibody 66885-1-Ig | Download protocol |
| IHC protocol for PRMT2 antibody 66885-1-Ig | Download protocol |
| WB protocol for PRMT2 antibody 66885-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Omega Biochemical Properties of CARM1: Impact on Western Blotting and Proteomic Studies | ||
Toxicol Appl Pharmacol Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5 | ||
Basic Clin Pharmacol Toxicol Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5. | ||
Cell Death Dis PRMT2 promotes RCC tumorigenesis and metastasis via enhancing WNT5A transcriptional expression | ||
Neurochem Int The antidepressant effects of protein arginine methyltransferase 2 involve neuroinflammation | ||
Int J Mol Sci Human C15orf39 Inhibits Inflammatory Response via PRMT2 in Human Microglial HMC3 Cell Line |
