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Species Reactivity
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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRMT1 Monoclonal antibody

PRMT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G8E2

Cat no : 68365-1-Ig

Synonyms

ANM1, HCP1, HMT2, HRMT1L2, IR1B4, PRMT1

Validation Data Gallery

Applications:
  • WB analysis using 68365-1-Ig

    WB analysis using 68365-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68365-1-Ig (PRMT1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inU2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PRMT1 Ready-To-Use IHC Kit. PRMT1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Sample-dependent, check data in validation data gallery

Product Information

68365-1-Ig targets PRMT1 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRMT1 fusion protein Ag29063 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 1
Calculated molecular weight 42 kDa
Observed molecular weight38~42 kDa
GenBank accession numberBC019268
Gene symbol PRMT1
Gene ID (NCBI) 3276
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

Protocols

Product Specific Protocols
WB protocol for PRMT1 antibody 68365-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols